Analysis of Sample Injection and Band-Broadening in Capillary Electrophoresis Microchips


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An implicit finite-volume numerical scheme has been developed to solve the three-dimensional transport equations that govern the electroosmotic and electrophoretic phenomena. The scheme consists of solving the ion transport equation and electro-neutrality condition coupled with Navier-Stokes equations for fluid flow in a sequential fashion until convergence is achieved. This capability is used for accurate modeling of electrokinetic focussing and of sample injection in cross-channels, and band-broadening in microchips. The simulations show that sample injection using electrokinetic switching technique produces sample plug with a large tail which has been observed in experiments. The simulations also show the sample plug broadening due to turns in the separation channel.

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Journal: TechConnect Briefs
Volume: Technical Proceedings of the 2000 International Conference on Modeling and Simulation of Microsystems
Published: March 27, 2000
Pages: 528 - 531
Industry sector: Sensors, MEMS, Electronics
Topics: Informatics, Modeling & Simulation, Micro & Bio Fluidics, Lab-on-Chip
ISBN: 0-9666135-7-0