tissue engineering – TechConnect Briefs

Hydrogel-based scaffolds for tissue engineering

Joshi S., Singh S.R., Alabama State University, US

Gels or hydrogels are described as the matrix of cross-linked polymers. Hydrogels are naturally a part of the body in the form of collagen, gelatin, mucous, tear films, cartilage, vitreous humor, cornea, and tendon. The [...]

A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair

Holmes B., Bulusu K., Plesniak M., Zhang L.G., Nanochon LLC, George Washington University, US

In recent years, 3D printing has become a popular and widely investigated method for the fabrication of large bone implants. 3D printed bone constructs, devices and treatments hold great potential to repair traumatic and chronic [...]

One and two dimensional inorganic nanoparticles’ interactions with fibroblasts and stem cells

Rashkow J.T., Talukdar Y., Lalwani G., Sitharaman B., Stony Brook University, US

We report the effects of one and two dimensional transition metal dichalcogenide nanoparticles, tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs), on fibroblastic cell (mouse fibroblasts (NIH-3T3) and human adipose derived stem cell (MSC)) [...]

Three-Dimensional Macroporous Carbon Nanotube Scaffolds for Stem Cell Expansion and Maintenance

Lalwani G., Gopalan A., D’Agati M., Patel S., Talukdar Y., Sitharaman B., Stony Brook University, US

Expansion of stem cells and maintenance of their self-renewal capacity in vitro requires specialized robust cell culture systems. Conventional approaches consisting of animal derived matrices and cocktail of growth factors have limitations such as consistency, [...]

2D Engineering of Protein-Based Nanoparticles for cell guidance

Tatkiewicz W.I., Seras-Franzoso J., García-Fruitós E., Vazquez E., Ventosa N., Ratera I., Villaverde A., Veciana J., Institut Ciencia Materials de Barcelona (CSIC)/CIBER-BBN, ES

Cells responses, like positioning, morphological changes, proliferation and apoptosis, are the result of complex chemical, topographical and biological stimuli. Here we show the macroscopic responses of cells when nanoscale profiles made with Inclusion Bodies (IBs) [...]

Biomimetic Polyurethanes for Regenerative Medicine

Ciardelli G., Sartori S., Serafini P., Boffito M., Caporale A., Silvestri A., Bernardi E., Boccafoschi F., Politecnico di Torino, IT

Degradable linear polyurethanes from non toxic building blocks were prepared. Bioactive peptides were incorporated in polyurethane chains, in order to create a synthetic equivalent of the Extracellular Matrix, able to mimic natural tissue. Both cell [...]

Chitosan Film/Membrane as a Surface to Alter Brain Glioma Growth and Migration

Gao W.J., Wang Y.-H., Gu H.-Y., Jandhyam S., Dukhande V.V., Lai J.C.K., Lai M.B., Leung S.W., Bhushan A., Lai J.C.K., Lai M.B., Idaho State University, US

A hydrophilic polysaccharide derived from chitin, chitosan has gained increasing popularity in biomedical applications such as tissue engineering, wound dressing, and drug delivery because of its presumed biocompatibility. These applications were founded on chemical similarities [...]

In-vitro and In-vivo Screenings of Electrospun Polycaprolactone-Carrageenan Nanofibrous Scaffolds for Tissue Engineering

Basilia B.A., Robles A.P., Ledda K.A., Dagbay K.B., Mapua Institute of Technology, PH

Nanofiber scaffolds are potential materials in three-dimensional cell-culture media. Among the most common and attractive polymer used for this application is polycaprolactone (PCL). PCL is non-toxic, biodegradable and biocompatible synthetic aliphatic polyester. It is completely [...]

Enhancement of in vitro Capillary Tube Formation by Substrate Nanotopography

Bettinger C.J., Zhang Z., Gerecht S., Borenstein J.T., Langer R., Massachusetts Institute of Technology, US

Controlling the morphology and function of cells using substrate nanotopography is a phenomenon that can be utilized in a variety of fields including tissue engineering and regenerative medicine. In this work, we explored the possibility [...]

A Non-Contact Culture Model for Investigating Cell Signaling and Nanotoxicity

Gao W.J., Dukhande V.V., Jandhyam S., Wang Y.-H., Gu H.-Y., Lai J.C.K., Lai M.B., Lai J.C.K., Lai M.B., Leung S.W., Idaho State University, US

There is general agreement that in vitro cell model systems offer many advantages over in vivo model systems in elucidation of cell signaling and other mechanisms underlying a toxicant’s effect. While the 3-D cell models [...]