RNA – TechConnect Briefs

Large scale Manufacturing of Calcium Phosphate Nanoparticles for Medical Applications

Panagiotou T., Delphi Scientific, US

Calcium phosphate (CaP) nanoparticles find medical applications in a number of areas, including delivery of nucleic acid therapeutics and calcium-based tissue engineering. CaP is well-studied biocompatible, bioactive, and bioabsorbable material and is used extensively in [...]

Neutron Scattering for Biological Research: Progress at the Bio-SANS Beam Line

Qian S., Pingali S.V., Weiss K., Urban V., O'Neill H.M., Langan P., Oak Ridge National Laboratory, US

Structure-function relationships remain a critical theme in understanding many important biological processes regarding energy, disease and other applications. At Oak Ridge National Laboratory, home to two of the most powerful neutron sources for research High [...]

Dielectrophoretic Isolation and Detection of Cancer Related DNA and RNA Nanoparticulate Biomarkers from Blood and Plasma

Heller M.J., Marciniak J., Skowronski E., Sonnenberg A., University of California San Diego, US

The analysis of cell free circulating (cfc) DNA and RNA nanoparticulate biomarkers continues to become more widely used for cancer diagnostics and management. Generally, cfc-DNA for “liquid biopsies” is isolated form plasma using relatively long [...]

Amplified On-Chip Fluorescent and Colorimetric Detection of DNA and RNA Hybridization by Surface-Initiated Enzymatic Polymerization

Tjong V., Yu H., Hucknall A., Chilkoti A., Duke University, US

We present a new on-chip, isothermal signal amplification technique for direct detection of unmodified DNA and RNA on microarrays using terminal deoxynucleotidyl transferase (TdT), a template-independent DNA polymerase that catalyzes the sequential addition of deoxynucleotides [...]

Novel alternative, multistranded, plasmid, and helical transitional DNA and RNA microarrays: the next generation of nucleic acid microarrays

Gagna C.E., Lambert W.C., New York Institute of Technology, US

Novel multistranded and alternative DNA, RNA and plasmid microarrays have been developed that allow for immobilization of intact, nondenatured nucleic acids. Biomolecules are anchored onto a solid support and can move freely, while researchers experiment [...]

Infant HIV and Nanotechnology

Goel N., Sharma R., Pt BDS Post Graduate Institute of Medical Sciences, IN

Background: New methods have emerged in detection and monitoring HIV using nanotechnology techniques but HIV in infants got less attention. Purpose: We report here the Human Immunodeficiency virus (HIV) and Acquired Immune Deficiency Syndrome (AIDS) [...]

Electrophoretic RNA Transport Through Transmembrane Carbon Nanotubes

Zimmerli U., Koumoutsakos P., ETH Zürich, CH

We study electrophoretic transport of RNA through carbon nanotubes embedded in membranes using molecular dynamics simulations. % Doped and naked carbon nanotubes are inserted into a dodecane membrane and a dimyristoylphosphatidylcholine lipid bilayer and we [...]

Synthesis of new Metal Complex – Biomolecule Hybrids as Redox Sensors for Specific Nucleobases

Gasser G., Bond A.M., Graham B., Kosowski Z., Spiccia L., Monash University, AU

There are a number of redox-active supramolecular receptors described in the literature, but those containing ferrocene as a redox-active reporter group are the most frequently cited. It has been shown that modified ferrocenyl conjugates can [...]