labeling – TechConnect Briefs

Quantum dot (Qdot) labeling of gene expression analysis and single molecule imaging in neurons

Jeromin A., Riley Z., Byrnes E., Parry S., Smith S., Dee N., Jones A., Allen Institute for Brain Science, US

The Allen Brain Atlas1 is the first large scale atlas of gene expression in the mouse brain, using chromogenic in situ hybridization (cISH) of over 20,000 genes.Qdots have excellent potential for muliplex imaging of gene [...]

Quantum Dot Nanocrystals in Neurosciences

Pathak S., Cao E., Davidson M., Jin S., Silva G., UCSD, US

We have developed labeling methods using commercially available quantum dots specific to neurons and glia (Pathak S, in press) and have used these tools to label GFAP intermediate filament cellular bridges as well as integrin [...]

Labeling of Cells with Silver and Gold Host-Guest Nanocomposites

Balogh L.P., Lesniak W., Sinani V.A., Bielinska A.U., Sun K., Nair B.M., Waite E., Kariapper M.S.T., Khan M.K., Roswell Park Cancer Institute, US

Nanocomposites combine the properties of nano-sized inorganic guest atoms/molecules with their nanoscopic size polymer host. We have synthesized water-soluble, biocompatible, fluorescent, and stable nanocomposites with regulated surface charge and controlled size for cell labeling studies. [...]

Photo-Enhanced Luminescence in Bio-conjugated Quantum Dots for Ovarian Cancer Biomarkers

Dybiec M., Korsunska N.E., Zhukov P., Zhukov T., Ostapenko S., Sellers T., Zajac A., Zhukov P., Zhukov T., University of South Florida, US

Ovarian cancer is the most lethal gynecologic malignancy. This largely reflects the fact that approximately 75% of cases are detected at advanced stages of disease, when cure is unlikely. In contrast, 5-year survival for patients [...]