Saule R. – TechConnect Briefs

Dependence of the Energy of Electric Pulse Required to Electroporate the Cell on the Pulse Duration for Pulses from 50 ns to 500 ms

Saulis G., Bitinaitė A., Vytautas Magnus University, LT

To successfully implement the technology of food processing with pulsed electric fields (PEF), it is important to know what particular electric treatment (pulse duration, field strength, etc.) would require the minimum energy. Here, theoretical relationships [...]

Estimation of the Energetic Barrier to Pore Formation and the Pore Radius Corresponding to this Barrier for Different Cell Lines

Bitinaitė A., Saulis G., Vytautas Magnus University, LT

The permeability of the cell membrane can be modified by exposing of cells to high-voltage electric pulses leading to the formation of nanometer-sized pores in the cell membrane (electroporation or nanoporation). This phenomenon is widely [...]

Determination of the Electroporation Threshold for pulses from 95 ns to 20 us

Saulis G., Balevicius S., Saule R., Stankevic V., Zurauskiene N., Vytautas Magnus University, LT

The dependences of the fraction of electroporated mouse hepatoma MH-22A cells on the pulse intensity were obtained for the cells exposed to single square-wave electric pulses with the durations from 95 ns to 20 us. [...]

Size of the Pores Created by an Electric Pulse: Microsecond vs Millisecond Pulses

Saulis G., Saule R., Vytautas Magnus University, LT

Modeling studies have revealed that the pulse of nanosecond-duration should create smaller pores than the pulse of micro-millisecond duration. It can be expected, that similar differences should also exist between the pore populations created by [...]