Formation of Giant Unilamellar Vesicles from Block Copolymers and Study of AqpZ and KvAP Protein Incorporation with Light Microscopy

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We have formed giant unilamellar vesicles (GUV’s) using two types of block copolymers—PMOXZ-PDMS-PMOXZ triblock and PBD-PEO diblock—and studied incorporation of labeled, transmembrane proteins using aquaporin (AqpZ), a water channel protein, and KvAP, a voltage-gated potassium [...]