Formation of Giant Unilamellar Vesicles from Block Copolymers and Study of AqpZ and KvAP Protein Incorporation with Light Microscopy
We have formed giant unilamellar vesicles (GUV’s) using two types of block copolymers—PMOXZ-PDMS-PMOXZ triblock and PBD-PEO diblock—and studied incorporation of labeled, transmembrane proteins using aquaporin (AqpZ), a water channel protein, and KvAP, a voltage-gated potassium [...]