Lim S.H. – TechConnect Briefs

Conjugation of DNA to Streptavidin-coated Quantum Dots for the Real-time Imaging of Gene Transfer into Live Cells

Kim J.K., Lim S.H., Lee Y., Shin Y.S., Chung C., Yoo J.Y., Chang J.K., Digital Bio Technology, Co, KR

We have developed the method for the conjugation of biotinylated DNA to streptavidin-coated quantum dots (QDs). QD-DNA conjugates and a highly sensitive fluorescence imaging technique are adopted to visualize gene transport across the membrane of [...]

Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip

Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]