A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis
Lattuada D., Roda B., Pignatari C., Magni R., Cattaneo A., Bolis G., Reschiglian P., University of Bologna, IT
HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]