US – Page 424 – TechConnect Briefs

Electrically Triggered Hydrogels: Mathematical Models and Simulations

Chatterjee A.N., De S.K., Aluru N.R., University of Illinois, US

Environmentally sensitive hydrogels have enormous potential in various applications. Ionic polymer gels have been shown to respond to a number of different stimuli like pH and electric field. One of the major advantages of electro-responsive [...]

A Micro Cantilever-based Pathogen Detector

Weeks B.L., Camarero J., Noy A., Miller A.E., Stanker L., De Yoreo J.J., Lawrence Livermore National Laboratory, US

Increasing efforts have been put into the developments of cantilever-based micro sensors. These devices show fast responses, high sensitivity, and are suitable for mass production. Currently, they are mainly applied for quality and process control, [...]

Macromolecular Crystallization in Microfluidics for the International Space Station

Monaco L.A., Spearing S.F., Morgan Research Corporation, US

Macromolecular Crystallization in Microfluidics for the International Space Station At NASA’s Marshall Space Flight Center in Huntsville, Alabama, we have begun the development of scientific hardware for macromolecular crystallization on the International Space Station (ISS). [...]

A Homogenous Small Molecule Microarray for Enzymatic Assays

Ma H., Wang Y., Pomaybo A.S., Tsai C., Schmidt S.P., Morewood Molecular Sciences, Inc., US

Small molecule microarray as an emerging technology promises to play a pivotal role in chemical genetics. However, in spite of the fast development of this technology special requirements, such as the immobilization of small molecules [...]

Assembly of Oriented Virus Arrays by Chemo-Selective Ligation Methods and Nanolithography Techniques

Camarero J.A., Cheung C-L, Lin T., Johnson J.E., Weeks B.L., Noy A., De Yoreo J.J., Lawrence Livermore National Laboratory, US

Many experimental techniques in biology and biophysics, and applications in diagnosis and drug discovery, require proteins immobilized on solid substrates. In fact, the concept of arrays of proteins attached to a solid support has attracted [...]

Immunosensing Using Fabricated protein Microarrays

Howell S.W., Inerowicz H.D., Guirl L., Regnier F., Reifenberger R., Purdue University, US

Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning [...]

Microfluidic Technology Applied to Protein Sizing and Quantitation

Barthmaier P., Agilent Technologies, US

Although protein analysis technologies are developing fast, the current standard method is still denaturing gel electrophoresis (SDS-PAGE). Many researchers rely on this traditional method that has not substantially changed in the past 30 years. However, [...]

Molecular Recognition on Demand

Sherrill C., Marshall D., Richmond C., Scherrer C., Prudent J.R., EraGen Bioscience, Inc., US

DNA is the only chemistry that allows for ?molecular recognition on demand?. That is, unlike any other molecular recognition chemistry, DNA allows for the simple design and rapid synthesis of molecule sets that will recognize [...]

Nanoscale Bio-Molecular Control Using EC-OWLS

Bearinger J.P., Vörös J., Spencer N.D., Hubbell J.A., Textor M., ETH Zürich/ Lawrence Livermore National Laboratory, US

A new technique has been developed that combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. This technique, termed Electrochemical Optical Waveguide Lightmode Spectroscopy (EC-OWLS), proved efficient in monitoring molecular surface adsorption and [...]

Freeze-fracture Electron Microscopy on Nanostructures for Drug & Gene Delivery

Papahadjopoulos-Sternberg B., NanoAnalytical Laboratory, US

The potency of drug/gene-loaded carriers is frequently depending upon their morphology adopted in a biological relevant environment. Freeze-fracture electron microscopy/1/ is not only a powerful technique to characterize drug/gene carrier on a nanometer resolution scale/2,4-11/ [...]