US – Page 380 – TechConnect Briefs

High Speed Processing of Large Amount of Bio-Nano Imaging Data for Cancer Research

Wang G., Wang M., Wang G., Wang M., Georgia Institute of Technology and Emory University, US

Bionanotechnologies such as quantum dots (QDs) or molecular beacons have generated huge amount of nanoimaging data to study in vitro and in vivo cell behavior for disease early diagnosis and therapeutic drug target. These images [...]

Mesoporous Silica for Desorption-Ionization Mass Spectrometry

Dattelbaum A., Iyer S., Los Alamos National Laboratory, US

Here we demonstrate the use of mesoporous silica thin films for matrix free laser desorption/ionization mass spectrometry (MS). In spite of the growing acceptance of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for biomolecular analysis, [...]

Formation of Polymer Nano-particles in Supercritical Fluid Jets

Gokhale A.A., Khusid B., Dave R.N., Pfeffer R., New Jersey Institute of Technology, US

The supercritical antisolvent process is an emerging technology for the production of micro- and nanometer-sized particles for a wide variety of bio-medical pharmaceutical applications. In this method, an organic liquid solution of the compound of [...]

Self-Assembly of Fluorescent Silica (glass) Beads

Kievesky Ya., Sokolov I., Clarkson University, US

We report self-assembly of fluorescent silica (glass) bids. The bids are a few microns glass nanoporous particles which contain florescent dyes imbedded in the pores. The pores are self-closed, so the dye does not leak [...]

Fluorescent Silica Beads for Detection of Cervical Cancer

Iyer S., Kievesky Ya., Woodworth C.D., Sokolov I., Clarkson University, US

We present the use of self-assembled fluorescent silica (glass) bids for detection of cervical cancer. The cells from three different individuals (3 normal and 3 tumor) were tested for affinity by using the bids, a [...]

Liposome Encapsulation of Fluorescent Nanoparticles: Quantum Dots and Silica Nanoparticles

Chen C-S, Yao J., Durst R.A., Cornell University, US

Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes by [...]

Labeling and Imaging of Human Mesenchymal Stem Cells with Quantum Dot Bioconjugates during Proliferation and Osteogenic Differentiation in Long Term

Shah B., Clark P.A., Stroscio M., Mao J.J., University of Illinois at Chicago, US

Tissue engineering approaches have the potential to repair damaged bone using a patient’s own mesenchymal stem cells (hMSCs). The objective of this study was to determine the feasibility of long-term labeling of hMSCS with semiconductor [...]

Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection

Yeh H.C., Ho Y.P., Wang T.H., John's Hopkins University, US

We describe a novel semiconductor quantum dot (QD)-based biosensing assay for single nucleotide polymorphism (SNP) detection. By integrating a QD-mediated dual-color fluorescence coincidence detection method with oligonucleotide ligation assay (OLA), we have achieved rapid SNP [...]

Strong DC and Low-Frequency AC Fields for the Manipulation of Particles and Fluids in Microfluidics

Kumar A., Qiu Z., Khusid B., Yeksel M., Acrivos A., New Jersey Institute of Technology, US

Electroosmosis and electrophoresis are considered to be the main phenomena for transport of aqueous liquids and particles in microfluidic devices because field-based techniques do not require any moving parts and can be incorporated more favorably [...]

A Microfluidic System for Continuous Magnetophoretic Separation of Suspended Cells Using Their Native Magnetic Properties

Han K-H, Bruno Frazier A., Georgia Institute of Technology, US

This paper presents the characterization of a continuous magnetophoretic microseparator for separating white and red blood cells from whole blood by using a high gradient magnetic separation method and microfabrication technology. The magnetophoretic microseparator directly [...]