Pala R., Nauli S.M.
Chapman University and University of California Irvine, US
Keywords: live cell imaging, magnetism, mechano-sensory, nanoparticles, Pkd2 mice
In the present studies, we designed a cilia-targeted delivery system to deliver fenoldopam (FD) specifically to the primary cilia. Hence, we devised the iron oxide nanoparticle (Fe2O3-NP)-based technology for ciliotherapy. Dynamic light scattering measurements showed that upon surface functionalization, the size distributions were increased from 102±3.8 to 126±4.6 nm. When the surface charge of the particles was analyzed, the charge repulsion increased after every functionalization step from +12.9±2.8 to -27.9±3.4. The successful formation of bare NPs and their surface functionalization were also examined by collecting X-ray diffraction and X-ray photoelectron spectra. Live imaging confirmed that the Fe2O3-NPs specifically targeted primary cilia in cultured cells in vitro and vascular endothelia in vivo. Importantly, the Fe2O3-NPs enabled the remote control of the movement and function of a cilium with an external magnetic field, making the non- motile cilium exhibit passive movement. When used in animal models, Fe2O3-NPs showed significant advantage with reduced side effect compared to FD alone.
Journal: TechConnect Briefs
Volume: TechConnect Briefs 2019
Published: June 17, 2019
Pages: 385 - 388
Industry sector: Medical & Biotech
Topics: Biomaterials, Diagnostics & Bioimaging
ISBN: 978-0-9988782-8-7