Topic: Biomaterials
Evaluation of Electric Impedance Spectra for Single Bio-Cells with Microfluidic Devices using Combined FEMLAB/HSPICE Simulated Models
Senez V., Yamamoto T., Poussard B., Fukuba T., Capron J.M., Fujii T., LIMMS/CNRS-IIS - University of Tokyo, JP
This paper describes a simple method to predict the electrical impedance spectrum of single & cultured cells in micro-devices. It can be used for the rapid design of micro-sensors as well as for more fundamental [...]
Amplification of Protein Adsorption on Micro/Nanostructures for Microarray Applications
The fabrication and operation of biodevices require the accurate control of the concentration of the bioactive molecules on the surface, as well as the preservation of their bioactivity, in particular proteins that have a significant [...]
Development and Characterization of a 2-D microchip-based Protein separation system
Feng J.J., Olazábal V, Olivares J.A., Sundaram S., Krishnamoorthy S., Iyer S., Los Alamos National Laboratory, US
Traditional methods in proteomics have relied on gel-based separation of proteins followed by directed cleavage of these proteins and mass spectrometry to measure the products. The emphasis on reliable, high-throughput analyses has led to the [...]
Molecular Simulation of DNA Microarrays – an Application of 2D particle mesh Ewald algorithm
Watanabe T., Kawata M., Nagashima U., National Institute of Advanced Industrial and Science and Technology, JP
We have developed the two-dimensional particle mesh Ewald (2D-PME) algorithm for molecular dynamics (MD) simulation, and applied it to have knowledge of the behavior of DNA and its interaction on microarrays, which crucial in the [...]
Dry Microarrays: A Generalized Data Source for Genome and Proteome Analysis
Microarray analysis methods can be applied to characteristic data obtained from biological sequences in matrix form (which could include data from experiments or bioassays as well). The rows correspond to a genome region (intron or [...]
Dielectrophoresis (DEP) of Cells and Microparticle in PDMS Microfluidic Channels
Dielectrophoresis (DEP) [1] The behavior of polarized, neutral particles to the non-uniform electric field has been successfully applied for the levitation and subsequent isolation of biological cells and other micron-sized inorganic particles [2, 3]. This [...]
An Injector Component Model for Complete Microfluidic Electrokinetic Separation Systems
This work presents a closed-form, numerically derived model of a key microfluidic electrokinetic separation system component, the cross injector. The model fits into a framework such that it can be combined with a network of [...]
Effect of Substratum Morphology on Cell Behavior
Contact guidance induced by the surface topography of the substrata influences the direction of cells with artificial material. Mouse bone marrow stromal fibroblast was examined on grooved substrata of varying dimension (0.3£gm~1.2£gm groove width, 0.5£gm [...]
Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip
Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR
Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]