Hastman D., Chiriboga M., Medintz I.
U.S. Naval Research Laboratory, US
Keywords: biosensing, catalysis, enzyme, nanoparticle, synthesis
The use of nanoparticle (NP) scaffolds for enzymatic systems has proven to be a useful tool for sensing specific enzyme activity as well as enhancing the catalytic rates of these systems. Although, NP-induced enhancement has been shown with a variety of different enzymes, this is not always the case, and the mechanism and magnitude of enhancement can drastically vary between enzymes. Using semiconductor quantum dots (QDs) as a model nanoparticle scaffold, we have examined a variety of single enzymes systems, which has provided a fundamental understanding of many of the processes involved in NP-induced enhancement. As we continue to probe such systems, we have started to implement QD-enzyme conjugates into complex multienzyme cascades, with the goal of preserving QD derived enhancement in the larger system. Here we discuss some of our this using QDs with single enzyme systems as well as our work to integrate QD-enzyme bioconjugates into more complex multienzyme systems.
Journal: TechConnect Briefs
Volume: TechConnect Briefs 2019
Published: June 17, 2019
Pages: 28 - 32
Industry sector: Advanced Materials & Manufacturing
Topics: Advanced Materials for Engineering Applications, Materials Characterization & Imaging
ISBN: 978-0-9988782-8-7