Ultra-High Performance Immunosensor Prepared by Glassy Carbon Electrode Modified with Nano Gold Particles and Antibody

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In previous reports, we have successfully fabricated biosensors that could measure chemical species that are important in environmental monitoring, as well as metabolites that are indicators of the proper functioning of our body. In our research group, we continue to expand our non-toxic sensor platform based prior reports to develop biosensors that are capable to measure metabolites at extremely low limits for biomedical applications, such as DNAs and biomarkers for cancer detections. As a demonstrative model, we are reporting an immunosensor for the detection of human immunoglobulin G (HIgG) which is the smallest but most common anti-infection antibody that makes up of majority (75-80%) of all antibodies in human body. The immunosensor consists of a carbon glassy electrode modified with a layer of biocomposite material, the biocomposite comprises coatings of polymer (cystamine/melamine), nano Au particles, and anti-HIgG. The detection range of 10-5 to 10-20 g/ml of this immunosensor is reported. The detection limit of this immunosensor is orders of magnitude more superior than any measurements reported in the literature. In addition, selectivity and stability of this biosensor will be reported.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: May 12, 2013
Pages: 45 - 48
Industry sector: Medical & Biotech
Topics: Diagnostics & Bioimaging, Sensors - Chemical, Physical & Bio
ISBN: 978-1-4822-0586-2