Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites

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Engineered multifunctional nanowires as novel biosensing tools for highly sensitive detection and Biosensors as innovative tools for detection of food borne pathogens and environmental virology have been well documented. Advanced applications require appropriate chemical functionalization of nanoparticles with organic molecules or their incorporation in suitable polymer matrices. Chitosan is a powerful chelating agent, which forms complexes with transition metals and heavy metals and shows antimicrobial activity. Present study explores in situ fabrication of chitosan-metal nanocomposites in view of their increasing applications as antimicrobial coating, wound dressing and antibacterial materials. Chitosan nanoparticles were prepared by ionic gelation and nanocomposites confirmed by FTIR, DSC, TEM; particle size of ~15nm. Chitosan-metal nanocomposites showed significant antibacterial activity against Staphylococcus aureus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro than component nanoparticles individually. Antifungal activity of chitosan-metal complex was also seen..Cytotoxicity studies conducted on Vero cell line (African green monkey kidney cell line), revealed good biocompatibility of nanocomposites as compared to metal nanoparticles. To date, this is the first report on cytotoxicity study of chitosan-copper and chitosan-ZnO nanocomposites. Chitosan-metal complexes can be a very promising candidate for novel antimicrobial agents for application in cosmetic, food, health care and textile industry.

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Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2014: MEMS, Fluidics, Bio Systems, Medical, Computational & Photonics
Published: June 15, 2014
Pages: 279 - 281
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4822-5827-1