Synthesis and characterization of FITC-aminated agarose as highly-fluorescent labeling agent

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A new fluorescent agent, FITC labeling agarose is designed to determine whether it can act as an effective fluorescent labeling agent of cell or not. The degraded agarose reacted with 3-chloro-1, 2-epoxypropane in NaOH medium for grafting epoxy group into agarose chain. The epoxy group containing agarose was aminated by ethylenediamine and then reacted with FITC to obtain FITC labeling agarose (FITC-AA). FITC-AA spontaneously formed nanoparticle at below 37C by gelling effect of agarose. IR analysis and EA proved that FITC was grafted to the aminated agarose (AA) successfully. Laser scattering experiment indicated that the size of FITC-AA nanoparticles decreased with the degradation time of agarose, and the mean diameter of FITC-AA could reach 159.2 nm when agarose was degraded for 24h. Zeta potential experiments disclosed that the zeta potential () of FITC-AA nanoparticles differed from each other with changes of their sizes, and  increased from about 8mV to 30.34 mV, corresponding to the mean size of FITC-AA nanoparticles decreasing from 1530.8 nm to 159.2 nm. Cell culture experiments confirmed that HL-60 cells and 3T3 fibroblast cells could be fluorescently marked by FITC-AA nanoparticle and their fluorescence sustained longer than FITC. After deposited for half a year, FITC-AA can dissolve in hot water and form nanoparticles when cooling, and its fluorescent properties remain. It is concluded that AA can be grafted by FITC and FITC-AA is an effective labeling agent for cells.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 157 - 160
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging
ISBN: 978-1-4398-3415-2