Jeromin A., Riley Z., Byrnes E., Parry S., Smith S., Dee N., Jones A.
Allen Institute for Brain Science, US
Keywords: gene expression, hybridization, in-situ, labeling, Qdot, quantum dot
The Allen Brain Atlas1 is the first large scale atlas of gene expression in the mouse brain, using chromogenic in situ hybridization (cISH) of over 20,000 genes.Qdots have excellent potential for muliplex imaging of gene expression due to their photstability and tenability. In addition, they can be conjugated to a variety of ligands for labeling. We will report on our progress to develop a high-throughput multiplex gene expression platform in brain, using riboprobes and quantum dots conjugated to various ligands. The signal intensity obtained with these probes is comparable to cISH and will allow to correlate the spatial expression pattern of genes. We will show an example, using Qdots for single-molecule imaging of ion channels in the membrane of living neurons.
Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 479 - 479
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4200-8504-4