Zhao Y., Zou M., Xue Q., Zhou P.
The Chinese Academy of Inspection and Quarantine, CN
Keywords: AIV, micro-channels, polystyrene microfluidic chip
AIV Ag of A type with viral nucleoprotein (NP), which covers all AIV subtypes, including high-pathogenic AIV was determined by polystyrene microfluidic chip ELISA method. In micro-channels of chip instead of conventional 96-well microtiter plates, a double-antibody sandwich ELISA (DAS-ELISA) was developed, in which rabbit Ab, mouse nuclear protein (NP) mAb and horseradish peroxidase-conjugated goat anti-mouse immunoglobin were used as coating, primary and secondary antibodies, respectively. Recombine Ag of AIV was prepared as positive control to ensure the on-site test safety. With TMB as substrate, AIV Ag could distinctly be judged by comparing colors of sample detection windows with that of the negative control in micro-channels, a preliminary result was obtained in accordance with conventional ELISA while total analytical time were within 1 h. The on-chip DAS-ELISA was well applicable to rapid and real-time screening of AIV. The sensitivity could be further improved by optimization experiments.
Journal: TechConnect Briefs
Volume: 3, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: May 20, 2007
Pages: 399 - 402
Industry sector: Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 1-4200-6184-4