Measurement of magnitude and frequency of cardiomyocyte contraction is significant not only for real-time monitoring of the toxicity of chemicals on cardiomyocyte, but also for understanding of the mechanisms of heart failure. Researches on measuring contractile force of cardiomyocyte have been performed in many research groups. Previously, we reported on the hybrid biopolymer microcantilever for measurement of contractile force of cardiomyocyte. PDMS cantilever was deflected vertically due to shear forces applied to cantilever structure by contraction of cardiomyocyte. Measuring these values of vertical deflection of cantilever, contractile force of cardiomyocyte was extracted through comparison with results of finite element method (FEM) simulations. We present new FEM model for contractile forces of cardiomyocytes on hybrid biopolymer microcantilevers more coincident with the contractile behaviors of real cardiomyocytes. In new model, the vertical deflection of cantilever was nearly linear to the portion of cardiomyocyte. The simulation results using previous FEM model were considerably different from analytical solution in tendency. However, the simulation results using new FEM model agreed well with analytical solution. We could see that FEM results for 2~5nN/um2 of contractile force using new model coincided very well with the experimental data of cantilever deflection, also.
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2008: Microsystems, Photonics, Sensors, Fluidics, Modeling, and Simulation – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: June 1, 2008
Pages: 737 - 740
Industry sector: Advanced Materials & Manufacturing
Topic: Informatics, Modeling & Simulation