NanoBio-Tools for Selective Activation of Toll-like receptors (TLRs)

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One of the emerging goals of nanotechnology is to functionalize inert materials to impart precise biological functions resulting in biocompatible nano-platforms. The exploration of the interaction between nanostructured materials and living systems is of fundamental and practical interest, and it opens new doors for a novel interdisciplinary research field. The innate immune response is the first line of defense against infectious disease. Toll-like receptor 3 (TLR3) and 9 (TLR9) are members associated with the specific recognition of double-stranded RNA (dsRNA) and unmethylated single-stranded DNA (ssDNA-CpG), respectively. Activation of TLR’s by their respective ligands induces MAPkinases cascades. In this contribution, magnetic metal oxides (MnO and –Fe2O3) nanoparticles were shielded using a multidentate functional copolymer and were biofunctionalized (ds RNA, ss DNA etc.). The biological specifity of these bionanotools was demonstrated by cell receptor specific binding in Human kidney cancer cells leading to the NF-kB activation and immuno modulated responses.The cells were imaged (MRI) using the magnetic property of the nanoparticles.

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Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 505 - 508
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4200-8504-4