Multifunctional Micropatterned Nanofiber Capable of Cell Patterning, Metabolite Detection, and Growth Factor Delivery

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This study describes the development of multifunctional nanofiber scaffolds consisting of multiple layers of nanofiber scaffolds and nanofiber-incorporated PEG hydrogels. We fabricated double-layered nanofiber scaffolds that were micropatterned with PEG hydrogels by combining two-step sequential electrospinning and hydrogel lithography. Each electrospinning process produced a nanofiber layer that was incorporated with different molecules, creating nanofiber scaffolds with vertically-differentiated functionality. Integration of non-fouling PEG hydrogel micropatterns into double-layered nanofiber scaffolds laterally divided nanofiber scaffolds into two different regions: (1) a cell or protein-repellent hydrophilic hydrogel and (2) a hydrophobic PS nanofiber , which allowed us to selectively control the position of cells and proteins. To demonstrate the possibility of scaffolds to carry out multiple functions simultaneously, we prepared micropatterned double-layered nanofiber scaffolds consisting of bare PS nanofiber as an upper layer and anti-albumin-immobilized nanofiber as bottom layer. We successfully demonstrated that the upper layer could be used to create micropatterns of HepG2 spheroids with uniform size, while the bottom layer could be used for in situ detection of albumin secreted by HepG2 spheroids in the upper layer. Finally, we immobilized growth factors and showed possibility of controlled release of growth factors from micropatterned nanofiber scaffolds

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 83 - 85
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4665-6276-9