Label-free Raman Micro-Spectral Imaging of the Microenvironment of PANC-1 Spheroids

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Spheroids are 3D tumor models, which reflect diverse properties of the growing tumor. Due to its avascular microenvironment, initial tumor growth patterns as well as subsequent tumor-core biochemistry may be studied in depth, from which insight into tumor mass differentiation and organization may be deduced. A label-free and non-destructive imaging method recently gaining recognition is Raman micro-spectroscopy. We have thus utilized the instrumentation in synergy with mathematical data-unmixing algorithms, which paint a biochemical picture of the spheroid milieu. This non-invasive method of analysis will ultimately give rise to a more in-depth comprehension of the biochemical organization of the spheroid, render a better understanding of tumor growth and development, elucidate drug penetration patterns, and subsequently offer insight into its suppression.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 13, 2011
Pages: 364 - 366
Industry sector: Medical & Biotech
Topics: Biomaterials, Cancer Nanotechnology
ISBN: 978-1-4398-7138-6