Injectable Glucan/Alginate/poly(γ-glutamic acid) Gel for Bone Formation and Differentiation

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Aqueous mixture of β-glucan, alginate and calcium form poly(γ-glutamic acid)(Ca-PGA) was compared with the traditional hydrogels cross-linking with calcium chloride and sodium form γ-PGA (Na-PGA). The suitable proportion by investigating the characters of swellability, pH sensitivity, retentiveness, the hemocompatibility of each hydrogels, compared with alginate/CaCl2 hygrogel one. MG63 osteogenic sarcoma cell lines exhibit many osteoblastic traits which are characteristic for bone forming cells, so we compare with various compositions and prepared by using MG63 cells. The results of hemocompatibility test showed no significant effect on the activated partial thromboplastin time (APTT) and thrombin time (TT) and minor adsorption of human serum albumin (HSA). The addition of glucan made the hydrogel more ductile than alginate with Ca-PGA one. This should be a useful phenomenon and can lead to the successful application while the hydrogel was injecting for the reconstruction of bone defects. Moreover, glucan can improve the growth of the cell. It helps the injectable hydrogel more efficiently. In vitro cytocompatibility results showed that Alg-CaPGA-Glu has more cell adhesion and mineralization than Alg only, PGA only, Alg-NaPGA, and Alg-CaPGA ones. Therefore, a biocompatible injectable matrix made of glucan, Ca-PGA and alginate could open up a clinical application to mankind.

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Journal: TechConnect Briefs
Volume: 1, Nanotechnology 2013: Advanced Materials, CNTs, Particles, Films and Composites (Volume 1)
Published: May 12, 2013
Pages: 678 - 681
Industry sector: Advanced Materials & Manufacturing
Topics: Advanced Materials for Engineering Applications, Coatings, Surfaces & Membranes
ISBN: 978-1-4822-0581-7