Formation of complex nanoparticles from a spherical protein (BSA) and two polycations (PDL with two molecular weights) using coacervation method of the polyelectrolytes

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Complex nanoparticles (CNPs) were formed by mixing Bovine Serum Albumin (BSA) and Poly-D-Lysine with low (LMW-PDL) and high molecular weights (HMW-PDL). Since BSA and both PDL biopolymers in neutral and alkaline pHs are oppositely charged biopolymers, so they can easily interact by physically mixing of them. This interaction was established by measuring the zeta potential of BSA and both PDL biopolymers before and after mixing, and the interaction process was called complex coacervation process. The minimum effective diameter, in the range of 200 ± 1.40 nm and 215 ± 1.97 nm, was achieved with LMW-PDL dissolved in phosphate buffer saline with 0.1 M NaCl at pH 7 and a mass ratio of 2.0 (BSA: PDL). These particle sizes decreased at 101 ± 7.02 nm nm with the addition of 0.25% glutaraldehyde as cross-linking agent, but polydispersity index (PDI) increased to 0.47 ± 0.07. Nanoparticles (NPs) from HMW-PDL showed effective diameter in the range of 250 ± 32.8 nm and 300 ± 24.4 nm; with the addition of 0.25% glutaraldehyde, the particle size decreased to 200 ± 1.75 nm and 220 ± 3.05nm, but PDI increased to 0.319 ± 0.008

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Journal: TechConnect Briefs
Volume: 1, Nanotechnology 2014: Graphene, CNTs, Particles, Films & Composites
Published: June 15, 2014
Pages: 291 - 294
Industry sectors: Advanced Materials & Manufacturing | Personal & Home Care, Food & Agriculture
Topicss: Advanced Materials for Engineering Applications, Personal & Home Care, Food & Agriculture
ISBN: 978-1-4822-5826-4