Detecting oxidation and reduction of Cytochrome c on Single Nanoparticles in Flow Using SERRS

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Surface enhanced Raman spectroscopy (SERS) is a powerful tool used for observing molecules and their structural properties, due to their ability to enhance the otherwise weak Raman scattering signals on a roughened metal surface or nanoparticles. By using an excitation source with a frequency matching the electronic transition of the analyte, signals can be further enhanced, the technique known as surface enhanced resonance Raman spectroscopy (SERRS). Recently, the detection of single nanoparticles under flow has been demonstrated where the time-limitations of non-flow environment were overcome by increasing the nanoparticle flux through the laser beam. Correlation spectroscopy also provides a method to detect single particles, where intensity fluctuations of the Raman bands from a small sample volume are studied. Here we aim to probe not only the presence of SER(R)S-active particles, but also the composition of the active layer, which is composed of reduced and oxidized cytochrome c (Cyt c). Using cyclic voltammetry, surface coverage of Cyt c on the electrode will be examined and these conditions for nanoparticle modification for controlled surface coverage. These particles will be examined with flow-based SERRS and correlation spectroscopy to extract chemical information and potentially reaction dynamics directly from the modified particles in microfluidic environment.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 13, 2011
Pages: 109 - 112
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging
ISBN: 978-1-4398-7138-6