Combined utility of dynamic light scattering and surface plasmon resonance characteristics for drug nanocarrier systems

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Study of plasmonic nanoparticles and their interaction with biomolecules has attracted great deal of attention due to potential utility in a wide variety of areas, such as drug nanocarrier systems. However, to enable full exploitation and deeper understanding of a hybrid system upon interaction, assessing both biomolecular and nanocarrier characteristics is of high significance. For nanoparticle-biomolecule interactions proteins can induce large scale changes on the surface of nanoparticles, such as dissolution, ion leaching, phase transformation and agglomeration. Herein, possible changes in characteristics of silver nanoparticles have been explored upon interaction with lysozyme, taking advantage of scattering and spectroscopic techniques. Silver nanoparticles (AgNPs) were synthesized and mixed with three different concentrations of Lysozyme, and the complex was characterized by UV-Vis, FTIR and DLS techniques. Results revealed that protein concentration plays critical role either in guiding formation of stable complexes or dictating irreversible aggregations of NPs. This investigation encourages combined utility of dynamic light scattering and surface plasmon resonance responses of nanoparticles as a facile approach for designing drug nanocarrier systems of optimized conditions.

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Journal: TechConnect Briefs
Volume: 1, Nanotechnology 2014: Graphene, CNTs, Particles, Films & Composites
Published: June 15, 2014
Pages: 452 - 455
Industry sector: Advanced Materials & Manufacturing
Topics: Materials Characterization & Imaging
ISBN: 978-1-4822-5826-4