Masuda J., Vig K.
University of Hawaii at Manoa, US
Keywords: 2D cell culture, 3D cell culture, antimicrobial peptide, bacteria inhibition, scaffold
The current study investigated two proprietary peptides, p359 and p753, for their antibacterial activity against Escherichia coli (E.Coli) and Staphylococcus aureus (S. aureus). Minimum Inhibitory Concentration (MIC) of bacteria for p359 and p753 was 50μg/mL and 25μg/mL respectively. MTT assay found p359 and p753 was not toxic to HEp-2 cells up to 100μg/mL. HEp-2 cells were infected with bacteria at Multiplicity of Infection (MOI) of 1:0.1 for 2D studies and 3D studies on 3D printed PLA scaffolds. Peptides p359 and p753 were added 24 hours after bacterial infection. MTT assay of the 2D cell culture found cells with peptide showed 80% more cell viability compared to the control. Live/dead assay and plate count of bacteria treated with peptides showed a 95% decrease in bacteria of 2D cell culture and a 70% decrease in bacteria of 3D cell culture versus without peptide. qPCR was performed on bacteria treated with peptide. There was a down regulation in gene expression of 16s, KatA, RecA, and grpE in E.coli and of SPY1258 in S.aureus with peptides compared to the control.
Journal: TechConnect Briefs
Volume: TechConnect Briefs 2019
Published: June 17, 2019
Pages: 381 - 384
Industry sector: Medical & Biotech
Topics: Biomaterials, Diagnostics & Bioimaging
ISBN: 978-0-9988782-8-7