Automated Image Processing and Analysis Tools for DNA Origami Nanostructure Characterization by Atomic Force Microscopy

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The DNA origami technique is highly utilized in the DNA nanotechnolgy field for its ability to self-assemble geometric architectures with near atomic precision. The structural fidelity will vary depending on the assembly reaction conditions. Therefore, researchers have relied heavily on atomic force microscopy (AFM) for structural characterization. In particular for its ease of use, high throughput capacity, and ability to resolve distances on the sub-nanometer scale. As a result, enormous data sets of DNA origami images are being generated faster and larger than can be analyzed within acceptable error. Computational approaches must be employed to parse and extract meaning from the data. Here, we discuss some common open-source software used to automate the processing and analysis of DNA structures in AFM images.

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Journal: TechConnect Briefs
Volume: TechConnect Briefs 2021
Published: October 18, 2021
Pages: 134 - 137
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Advanced Manufacturing, Materials Characterization & Imaging
ISBN: 978-0-578-99550-2