We exposed Escherichia coli K-12 to engineered silver nanoparticles (AgNPs) on to understand the antibacterial activities of AgNPs. Optical density measurement by UV-Vis was used to determine the growth inhibition of AgNPs and silver ions. Exposure to 10 mg AgNP/L as total Ag concentration showed the significant delay of lag phase. E. coli K-12 did not grow up when exposed to 30 mg AgNP/L as total Ag concentration. Silver nitrate was more toxic than AgNPs if they were compared as total Ag concentration. Transmission electron microscopy (TEM) was used to observe cell morphological changes and intracellular accumulation of AgNPs. AgNPs were found to interact with cell surface and significant amount of AgNPs were accumulated in E.coli K-12.
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 289 - 292
Industry sectors: Advanced Materials & Manufacturing | Energy & Sustainability
Topic: Environmental Health & Safety of Nanomaterials