Amplified On-Chip Fluorescent and Colorimetric Detection of DNA and RNA Hybridization by Surface-Initiated Enzymatic Polymerization
We present a new on-chip, isothermal signal amplification technique for direct detection of unmodified DNA and RNA on microarrays using terminal deoxynucleotidyl transferase (TdT), a template-independent DNA polymerase that catalyzes the sequential addition of deoxynucleotides [...]