Harper G. – TechConnect Briefs

Physico-chemical Structure of Multilayer Polyion Complexes Comprising PolyHyrdoxylAmine & Genomie DNA

Harper G., WetaScience, UK

Alternating multilayers of DNA and polyhydroxylamine [PT] were produced on cationic microbeads [C]. DNA elution on two enclosed layers and a surface layer of DNA, provided 10µg DNA/mg bead/ per layer and yield 54µg/mg bead. [...]

Synergistic Release of DNA from pH Responsive Polyion Complexes (PICs)

Harper G., WetaScience, UK

Polyelectrolyte complexes based on an outer layer of cationic polyhydroxylamine (PT) were prepared on carboxylated microparticles pretreated with polyamine (A) and a middle layer of Polyacrylic Acid (PA). A synergistic effect between PT and PA [...]

Cationic Nanoparticles based on pH Responsive Polyion Complexes containing Plasmid GFP DNA

Harper G., Gibbs R., WetaScience, UK

Cationic nanoparticles were formulated from a polycationic Polyhydroxylamine (PT) with low pKa and polyanionic PolyAcrylic Acid (PA) that form binary polyelectrolyte complexes (PECs). Particles spontaneously form on component mixing with sizes between 80-700nm and particle [...]