Caggana M. – TechConnect Briefs

A Micromachined Sparse Cell Isolation Device: Application in Prenatal Diagnostics

Mohamed H., Turner J.N., Caggana M., Wadsworth Center, New York State Department of Health, US

The isolation of fetal cells from maternal circulation is the subject of intense research. Fetal cells can be isolated using several enrichment methods, however no technique to specifically isolate and use them for genetic diagnosis [...]

Purification of PCR-Inhibitory Components by Cellulose Acetate Membranes

Mohamed H., Szarowski D.H., Lepak L.A., Spencer M.G., Martin D.L., Caggana M., Turner J.N., New York State Department of Health, US

Conventional methods for extracting DNA from blood results in the presence of heme, which is an inhibitory factor decreasing PCR sensitivity. Current techniques for removing the inhibitors are laborious, costly, and/or sample-specific. We investigated using [...]

Circulating Tumor Cells: Capture with a Micromachined Device

Mohamed H., Murray M., Turner J.N., Caggana M., New York State Department of Health, US

The isolation and analysis of circulating tumor cells (CTCs) from blood is the subject of intense research. Tests to detect metastasis are available but progress is hampered by the lack of tumor-specific markers and predictable [...]