Battaglia G. – TechConnect Briefs

Polymersomes as model of transcytosis across Blood-Brain Barrier (BBB)

Tian X., Cantón I., Battaglia G., Univ of Sheffield, UK

In this work, two formulations kinds of diblock copolymers ((poly(2- methacryloyloxy)ethyl phosphorylcholine)-co-poly(2-(diisopropylamino)ethyl methacrylate(PMPC25-‐PDPA70) and poly(ethylene glycol)-co-poly(2- (diisopropylamino)ethyl methacrylate)(PEO113-‐PDPA70))will be employed,blending them in series ratios in order to obtain the best morphology and size across the [...]

Polymersome macromolecule delivery across intact human skin

Pegoraro C., MacNeil S., Battaglia G., University of Sheffield, UK

Skin is a highly stratified and impermeable tissue that confers specific size, charge, hydrophilic and lipophilic constraints on the types of molecules that can diffuse across it. Here we report the use of a novel [...]

Polymersomes and Imaging: A Quantitative Approach

Joseph A.S., Brown N.J., Battaglia G., University of Sheffield, UK

Nano-sized carriers are widely used as imaging tools. Quantification of the amount of carrier delivered provides useful informations in development and testing stages. This work describes a HPLC method to quantify fluorescent labelled polymersomes. Polymersomes [...]

A Non-toxic Cellular Staining Agent Based on Fluorescently Labeled Poly[2-(methacryloyloxy)ethyl phosphorylcholine]

Warren N.J., Massignani M., Armes S.P., Battaglia G., Lewis A.L., The University of Sheffield, UK

The biocompatible monomer 2-(methacryloyloxy)ethyl phosphorylcholine (MPC) is polymerized by atom transfer radical polymerization (ATRP) using a fluorescent ATRP initiator and fluorescent comonomers. In this study we show that PMPC alone can be used as a [...]

Three-dimensional cell instructive scaffolds to direct stem cell fate

Viswanathan P., Chirasatitsin S., Reilly G., Engler A., Battaglia G., University of Sheffield, UK

Novel biomaterials design rarely incorporates the range of topographical (10nm – 100um) and chemical cues required to influence cell behaviour. Of particular interest is controlling human mesenchymal and embryonic stem cell fate. Recently it has [...]

Controlling pH responsive polymersome assembly

Pearson R., Battaglia G., University of Sheffield, UK

Size is an important parameter in delivery systems. It plays a part in whether a particle avoids filtration, evades immune cell binding and by which internalisation pathways are available. However, it is hard to obtain [...]