Müller U., Nisch W., Neugebauer S., Schuhmann W., Linke S., Kaczor M., Lohmüller T., Spatz J., Motz M., Sorsa J., Hecke S., Hartwich G., Stelzle M.
NMI Natural and Medical Sciences Institute, DE
Keywords: biosensor, diagnostics, nano-pore, nanosphere lithography, redox cycling
The primary objective of this research is to create electrode systems for use as electrochemical transducers with enhanced sensitivity in order to enable detection of low concentration diagnostic targets not accessible to date. NanoBioPore sensors consist of micro- or nano-porous metal-insulator-metal layer systems and exhibit an effective electrode spacing of only 100 nm. Patterning of pores was achieved by utilizing self-assembled particles as shadow masks during the deposition of the top electrode. Amplification factors > 50 have already been demonstrated, which is a significant improvement when compared to redox cycling factors reported from measurements using conventional interdigitated electrode (IDE) systems. A variety of biomolecules conjugated with alkaline phosphatase were immobilized in the pores and shown to be functional as evident from detection of p-aminophenol generated by the enzyme. In conclusion, high sensitivity combined with low fabrication costs due to the utilization of nanosphere lithography should open a broad range of applications for these sensors, particularly in point-of-care diagnostics.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 206 - 209
Industry sector: Medical & Biotech
Topic: Diagnostics & Bioimaging
ISBN: 1-4200-6183-6