Papers – Page 719 – TechConnect Briefs

Application of Quantum dots for the lifespan study of Caenorhabditis elegans

Huang G.S., Chang K-M, Chen Y-C, Yeh L-G, National Chiao Tung University, TW

The progress in identifying drugs that extend lifespan of Caenorhabditis elegans has been slow partly due to the tedious and labor-intensive nature of drug screening. We labeled C. elegans by mixing quantum dots, FITC, or [...]

In vivo imaging by luminescent carbon nanotubes

Shi D., Guo Y., Dong Z., Lian J., Wang W., Liu G., Ewing R., University of Cincinnati, US

There is currently an increasing need for early detection of cancer before anatomic anomalies are visible. A major challenge in cancer diagnosis is to be able to locally biomark the cancer cells in clinical pathology [...]

Synthesis, Encapsulation and Functionization of Composite Organic-Inorganic Nanoparticles with Distinctive Surface Enhanced Raman Signatures

Zhang J., Lu Y., Lutz B., Cao M., Qin H., Yamakawa M., Dentinger C., Nguyen L., Chmura A.J., Zhu J., Lutz B., Sung K-B, Swartz K., Wang L., Su X., Sun L., Sung K-B, Berlin A.A., Chan S., Sun L., Sung K-B, Intel Corporation, US

Composite organic-inorganic nanoparticle (COIN) is a new type of optical label for multiplex biomolecule detection. It has strong Raman signal intensity and potentials for a large number of unique Raman signatures. We have developed methods [...]

In Situ Investigation of Uptake Phenomena of Biological Molecules and Silica Nanoparticles into Mammalian Cells in Microstructures

Lee W.G., Bang H., Yun H., Kim J.A., Cho K., Shin Y.S., Chung C., Chung N.C., Chung C., Chung N.C., Chang J.K., Han D-C, Seoul National University, KR

Electroporation is a powerful gene transfection method to deliver foreign biological molecules such as DNA and protein into living cells with electric pulsations. However, conventional cuvette-type electroporation techniques generally suffer from poor transfection efficiency and [...]

Fluorescent Structural DNA Nanoparticles Functionalized With Phosphate-Linked Nucleotide Triphosphates

Williams J.G., Reynolds B.L., Keefe K., Anderson J.P., LI-COR Biosciences, US

Structural DNA nanoparticles functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were tested as a source of dNTPs for DNA polymerase. The particles were prepared by strand-displacement polymerization from a self-complementary circular template. An intercalating dye (SYBR-101) [...]

Comparison of the Efficiency of Different Methods for the Lysis of Cells in Lab-on-Chip Systems

Drese K., von Germar F., Roesser T., Hansen-Hagge T., Bullema J., Bolt P., TNO Science and Industry, NL

Many diagnostic or analytical tasks are based on detection of specific amplificates of DNA or RNA. Lab-on-Chip systems are developed for such tests because often only small sample volumes and analyte concentrations are available. A [...]

Diode Laser Generated Ultrasound for Human Blood Cell Lysis

Ussing T., von Gersdorff L.C., FluimediX, DK

The lysing of human blood leukocyte cells prior to performing e.g. DNA analysis is performed on a routine basis in most biochemical labs. Well-established techniques include using a lysis agent or using mechanical methods such [...]

Lab-on-chip for the isolation and characterization of circulating tumor cells

Laureyn W., Liu C., O’Sullivan C.K., Llaudet E., Nilsson O., Drese K.S., Roeser T., Ritzi M., Hauch S., Albert W., Borgen E., Nygren A.O.H., IMEC, BE

A consortium of 7 European partners has undertaken the challenging task to develop a microfluidic system (μTAS) capable of the isolation/enrichment and consecutive RNA/DNA characterization of circulating tumor cells (CTCs), to be used as a [...]

Noise-Differential Based Detection of Cells on a Microelectronic Sensor/Actuator Array

Manaresi N., Lanzetta F., Romani A., Medoro G., Guerrieri R., Silicon Biosystems spa, IT

We present a new method to count cells on a microelectonic lab-on-a-chip integrating an array of Dielectrophoretic (DEP) cages and embedded optical sensors. Cells are trapped by DEP cages above photodiodes measuring the optical near [...]

One-step Pathogen Specific DNA Extraction from Whole Blood On a Centrifugal Microfluidic Device

Cho Y-K, Lee B-S, Lee J-G, Lee Y., Park J.M., Lee B-S, Lee J-G, Lee Y., Lee B-S, Lee J-G, Lee Y., Ko C., Samsung Advanced Institute of Technology, KR

We report a fully integrated pathogen specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. Using the innovative Laser Irradiated Ferrowax Microvalves (LIFM) together with the rapid cell lysis method using [...]